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Release date：2018-12-24

JACI:

[IF:13.1]

Component-resolved diagnosis of house dust mite allergy with a large repertoire of purified natural and recombinant allergens from the major species of mites worldwide
DOI: https://doi.org/10.1016/j.jaci.2004.12.667
Abstract:
RATIONALE: Mites are one the most important sources of allergens associated with allergic diseases. Although immunotherapy is the only form of treatment that can alleviate these conditions, allergenic extracts used for such purposes are not well defined.
METHODS: The aim of this study was to evaluate the IgE binding profile of a series of patients attending allergy clinics in two different environments (Singapore and Italy) by IgE immuno-blotting using an arrayed panel of more than 100 different purified natural or recombinant allergens from major species of mites worldwide. This may contribute to improved selection of patients for immunotherapy with Dermatophagoides spp. and/or Blomia tropicals sensitization and may also be used for monitoring of patients undergoing treatment.
RESULTS: Majority of patients with sensitization to Dermatophagoides spp. could be diagnosed with a combination of the group 1, 2, 4, 5, 7 and 13 allergens while those with sensitization to Blomia tropicalis could be diagnosed with a combination of rBlo t 5, rBlo t 5 paralogue, rBlo t 13, rBlo t 13 paralogue, Blo t 3 and Blo t 12. The group 10 (tropomyosin) mite allergens however did not correlate with sensitization to several other tropomyosin allergens e.g., from seafood or cockroach.
CONCLUSIONS: We could discriminate mite-allergic patients who were mainly sensitized to the major Dermatophagoides allergens (group 1 and 2) from patients with broad sensitization. Different allergens are needed to diagnosed patients found in Dermatophagoides-predominant regions (e.g., Italy) and where Blomia tropicalis is predominant (e.g., Singapore).
All Author:
A.S.L. Tay, H.S. Shang, X.Z. Bi, K. Reginald, Y.F. Gao, S.T. Ong, A.C. Angus, W.L. Wang, K.T. Kuay, D.Y. Wang, A. Mari, F.T. Chew

2018-12-24 Article

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杭州浙大迪迅生物基因工程有限公司

Release date：2018-12-24

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杭州浙大迪迅生物基因工程有限公司

Release date：2018-12-24

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